Titre : An assessment of a massively parallel sequencing approach for the identification of individuals from mass graves of the Spanish Civil War (1936-1939)
Conférencier : Ferran Casals (Genomics Core Faciliy, CEXS – Universitat Pompeu Fabra, Barcelona)
His current research interest include the development and application of new experimental methodologies for genetic analyses, the study of the genetic etiology of rare diseases, and the analysis of the genetic variation across human populations, with particular interest in rare functional variants and its relation to disease prevalence. At the Genomics Core Facility we also develop collaborative projects with other UPF laboratories, covering a variety of applications of next-generation sequencing technologies to different research fields.
Résumé
Next generation sequencing technologies have opened new opportunities in forensic genetics. Here we assess the applicability and performance of the MiSeq FGx™ & ForenSeq™ DNA Signature Prep Kit (Illumina) to the identification of individuals from the mass graves of the Spanish Civil War (1936-1939). The main limitations for individual identification are the low number of possible first-degree living relatives and the high levels of DNA degradation reported in previous studies. Massively parallel sequencing technologies enabling the analysis of hundreds of regions and prioritizing short length amplicons constitute a promising tool for this kind of approaches. In this study, we first explore the power of this new technology to detect first and second degree kinship given different scenarios of DNA degradation. Second, we specifically assess its performance in a set of low DNA input samples previously analyzed with capillary electrophoresis technologies. We conclude that this methodology will allow identification of up to second-degree relatives, even in situations with low sequencing performance and important levels of allele drop-out; it is thus a technology that resolves previous drawbacks and that will allow a successful approximation to the identification of remains.
Conférence organisée conjointement par le Centre international de criminologie comparée et le département d'anthropologie.
